Human erythrocytes on the regularity of carbohydrate intake.docVIP

 PAGE \* MERGEFORMAT 12 Human erythrocytes on the regularity of carbohydrate intake Author: power on waves, Lu Tracy, Min-Xia, Hu Wenbo, Wang Yan, Han Ying Abstract lyophilization of human red blood cells in clinical application of great significance. A number of sugars, especially trehalose, can improve the number of low biological or cell tolerance to dry conditions, but how the sugar into cells is a challenge. This study investigated the human erythrocyte on the regularity of carbohydrate intake. At different temperatures (4,25 and 37 ℃), different concentrations (0,0.2,0.4,0.6,0.8,1 mol / L) and different incubation time (1,3,5,7,9 hours) under the conditions tested RBC pairs of trehalose and glucose absorption rate and the amount of free hemoglobin and red blood cells were determined variability index. The results showed that: With the temperature rise and the increase in extracellular glucose concentration, erythrocyte sugar absorption rate will also rise, the intracellular trehalose and glucose concentrations up to 30 mmol / L and 40 mmol / L or more. But the incubation time on the trehalose and glucose absorption rate affected differently, with time, the concentration of intracellular trehalose was first elevated and then decreasing trend, while the glucose uptake rate was steadily rising. But the sugar absorption process right of free hemoglobin and red blood cell deformation have a negative impact, especially trehalose, which comes mainly from osmotic injury. Conclusion: The erythrocyte sugar absorption rate and incubation temperature, exogenous glucose concentration and incubation time of close, but under certain conditions, the sugar absorption efficiency was also higher, but this process on a certain red blood cell damage, which may affect the sugar class lyophilization of red blood cell research application prospects. Future studies should focus on how to deal with cell injury and the relationship between glucose absorption efficienc