Human erythrocyte 20S proteasome characterization and proteomics 20S proteasome from different sources of heterogeneity.docVIP

 PAGE \* MERGEFORMAT 18 Human erythrocyte 20S proteasome characterization and proteomics 20S proteasome from different sources of heterogeneity Of: Zhang Jing Deng Yanchun Li Zhili Liu Hui Kwok-keung [Abstract] through the integration of differential centrifugation and polyacrylamide gel electrophoresis (native PAGE) technique, the establishment of effective separation of 20S proteasome (20S core particle, CP method. Comparing with the traditional purification methods, this method of economic and rapid characteristics of different tissues and cells can be separated from the CP. use this method on human red blood cells were the source of the CP subunit 2 DE separation and MALDI TOF / TOF MS identification. The results showed that 33 could be identified with different molecular weight and isoelectric point of proteins, this number is far greater than the 14 CP subunits. In addition, the use of non-denaturing / denaturing sodium dodecyl sulfate polyacrylamide gel electrophoresis (native / SDS PAGE technique compared from yeast, mouse liver, human red blood cells, human pancreatic cancer cell line SW1990 and PANC 1 of the CP and subunits of the differences in the electrophoretic behavior, the heterogeneity of the proteasome. [Keywords:] proteasome, heterogeneity, non-denaturing polyacrylamide gel electrophoresis, differential centrifugation, cells 1 Introduction 20S proteasome (20S core particle, CP in different ways related to other proteasome activator, such as the 19S regulator particles, 11S particles adjustment (also known as PA28 and PA200 binding (not specified, the following term “proteasome” includes CP and CP combined with the different activators to form complexes, mediated by intracellular protein degradation and involved in cell cycle regulation, DNA repair, chromosome stability, transcriptional activation, signal transduction and antigen presentation and other processes [1 3] . CP is a molecular weight of about 700 kDa protein complex