Human HLA-DP gene polymorphism and ulcerative colitis-associated research.docVIP

 PAGE \* MERGEFORMAT 5 Human HLA-DP gene polymorphism and ulcerative colitis-associated research Author: Guo Jian Liu Ruo-Dan Zhou Jing Liu Xinmin Liu Ying Xiong Jianping Hu Yin-Ching [Abstract] Objective: To explore the genetic level from ulcerative colitis (UC) in the pathogenesis and analyzed with anti-neutrophil cytoplasmic antibodies (ANCA) and disease sub-type relationship. Methods: Indirect immunofluorescence assay were 86 cases of UC patients and 128 healthy persons serum ANCA testing, and polymerase chain reaction - restriction fragment length polymorphisms detected by oligonucleotide typing Shenzhen Han HLA-DPB1 gene polymorphism, using PCR technology for HLA-DPB1 gene exon 2 (exon 2) in vitro amplification; then use the Ban II, Fok I, Dde I, Rsa I, EcoN I, BstUI 6 kinds of restriction in enzyme digestion of amplified products, electrophoresis separation of fragments, according to the pattern of fragments to determine the corresponding genotype. Results: UC patients with ANCA positive rate was 53.5% in the control group were negative; UC patients with HLA-DPA1 allele frequency (0.49) and the control group (0.18), the difference was significant (P = 0.0032, RR = 5.681 , Pc = 0.033). Conclusion: HLA-DPA1 and the positive rate of ANCA in UC were significantly increased, HLA-DPA1 and ulcerative colitis have a certain association between ulcerative colitis patients and normal subjects there are differences in the immune genetic heterogeneity. [Keywords:] Ulcerative colitis; anti-neutrophil cytoplasmic antibody; HLA-DPA1 gene; polymerase chain reaction; restriction fragment length polymorphism 〔 Abstract〕 Objective To study HLA-DPA1 genotyping and to analyze the relationship among HLA-DP genotyping, antineutrophil oplasmic antibodies (ANCA), clinical classification of ulcerative colitris (UC). Methods Eighty-six UC patients, 128 healthy subjects (HS) were enrolled, and serum ANCA with indirect immunoflorecence and HLA-DPAl genotypes were examined in 86