Human IL12 Construction of plant expression vector.docVIP

 PAGE \* MERGEFORMAT 9 Human IL12 Construction of plant expression vector [Abstract] Objective: To construct human interleukin  12 (hIL  12) gene plant expression vector. Methods: PCR technology from plasmid pCA13  hIL  12 to amplify the hIL  12 genes, SmaI, SacI digested hIL respectively  12 genes and the plant expression vector pBI121, recycling, T4DNA ligase a recombinant plasmid pBI121  hIL  12, double-enzyme digestion and DNA sequencing identified, through the three pro-hybridization to vector pBI121  hIL  12, respectively into Agrobacterium Agrobacterium tumefaciens EHA105 and GV3101, were identified by PCR method. Results: double digestion and DNA sequencing confirmed that hIL  12 has been inserted into the expression vector pBI121 in, PCR amplification showed that the recombinant expression vector pBI121  hIL  12 successfully transferred to Agrobacterium tumefaciens EHA105 and GV3101 in. Conclusion: The successful construction of the plant expression vector pBI121  hIL  12, further use of transgenic plants for the production of hIL  12 laid the experimental foundation. [Keywords:] human interleukin  12 Rhizobium plant expression vector is a vector 0 Introduction Human interleukin  12 (hIL  12) can promote natural killer cells (NK) and T cell proliferation, activation and cell toxicity induced production of IFN-γ , regulate Th0 cells to Th1 cell differentiation and proliferation, enhance cell-mediated immunity and other biological activity [1], in the body’s anti-tumor, anti-virus, anti-allergy played an extremely important role in [2]. a natural state, in vivo IL  12 is low, get more difficult. we have built hIL  12 gene plant expression vector for the use of plant genetic engineering technology to produce hIL  12 genes in the preparatory work. 1 Materials and methods 1.1 Materials E. coli strain DH5α , containing human IL  12 plasmid pCA13  hIL  12 from Zunyi Medical College of Biochemistry and Mo