Human lung adenocarcinoma cell line A549 cells two-dimensional electrophoresistime of flight mass spectrometry.docVIP

 PAGE \* MERGEFORMAT 22 Human lung adenocarcinoma cell line A549 cells two-dimensional electrophoresistime of flight mass spectrometry Author: Yang Shuan-ying, TIAN Ying-election, Nanyan East, Bu Lina Huo Shu-fen, Ruan Yu Song [Abstract] Objective preliminary analysis of human lung adenocarcinoma cell line A549 cell protein expression, from the proteome level to explore the molecular mechanism of pathogenesis of lung adenocarcinoma. Methods 2  DE technology on human lung adenocarcinoma cell line A549 cells, total protein were separated to obtain protein expression profile; application of matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI  TOF  MS) combination of bioinformatics for protein identification. The results of three adhesive protein spots on average number of 1138 ± 49, average matching points for the 986 ± 32, matching the rate of 85.8%. Randomly selected background clearly distinguish the higher protein content of 20 spots for MALDI  TOF  MS analysis, were obtained 18 peptide mass fingerprint (PMF). The PMFs quality data through the Aldente software SWISS  PROT database queries, according to matching fragments and amino acid sequence coverage and so on, initially identified 15 kinds of proteins. According to function, these proteins can be divided into: ① basic metabolism-related enzymes: fructose-2  phosphate aldolase, depending on the network aldehyde dehydrogenase 1 (RALDH1), pyridoxine kinase aldehyde; ② cytoskeleton categories: protein, cytokeratin 8 (CK8), β  2 chain, tubulin; ③ stress-related proteins: Protein disulfide isomerase A3 (PDIA3); ④ signal transduction molecule: membrane associated protein 1 (ANX1), annexin 4 ( ANX4); ⑤ molecular chaperone: heat shock protein 60 (HSP60), heat shock protein β  1, inhibin (PHB); ⑥ transcription and translation-related proteins: heterogeneous ribonucleoprotein H (hnRNP H); ⑦ Miscellaneous : Rgr protein, NPM, PCBP1. Conclusion 2  DE / MAL