Human fetal lung mesenchymal stem cells on the repair of lung injury.docVIP

 PAGE \* MERGEFORMAT 13 Human fetal lung mesenchymal stem cells on the repair of lung injury Study: East Qian Ming Peng Liping Liu Qian [Abstract] Objective To study the human fetal lung mesenchymal stem cells (MSCs) on the repair of lung injury. Methods in vivo and in vitro cell culture experiments to observe the human MSCs on the pulmonary epithelial cell growth regulation. MSCs results of human fetal lung can effectively control the growth of the alveolar epithelium. Conclusion human fetal lung MSCs and their conditioned medium can reduce the human lung epithelial injury and accelerate repair, and thus for clinical treatment of COPD, viral pneumonia, lung injury caused by the provision of new biological therapies. [Keywords:] people; fetal lung; mesenchymal stem cells; damage lung tissue Human fetal lung mesenchymal stem cells (MSCs) were cultured in vitro can be spread over 17 generations, the morphology and growth characteristics are not changed significantly in the G0/G1 phase of the cell number, with the typical characteristics of stem cells, The phenotype similarities with bone marrow-derived MSC (1), can be induced to differentiate into bone cells, fat cells and other cells, for the treatment of lung injury and proposed a new way of thinking. In this study, in vivo and cell culture experiments, human fetal lung MSCs observed in lung epithelial cell growth regulation, confirmed in lung injury, human fetal lung MSCs and their conditioned medium can reduce the human lung epithelial injury and accelerate repair, and thus for clinical treatment of chronic obstructive pulmonary disease (COPD), viral pneumonia and other lung injury caused by the provision of new biological therapies. 1 Materials and methods 1.1 MSC regulation of lung epithelial cell growth in vivo experimental animals: use of immune rejection-free NOD / SCID mice and the preparation of airway epithelium, alveolar epithelial injury model .NOD / SCID mice after exposure to 137