Hyaluronic acid oligosaccharides reverse MCF- 7-ADM resistance and pro-apoptotic effect of.docVIP

 PAGE \* MERGEFORMAT 5 Hyaluronic acid oligosaccharides reverse MCF? 7/ADM resistance and pro-apoptotic effect of Author: Wang Liping Bao-Zhen Sui Xia Zhao Chunting Nakatsukasa shipping [Abstract] [Purpose] To study the reversal of hyaluronic acid oligosaccharides MCF? 7/ADM cells, the role of drug resistance. [Methods] MTT Study of hyaluronan oligosaccharides on the MCF? 7/ADM reversal effect, flow cytometry cell-surface resistance-associated protein P? Gp, MRP expression and apoptosis regulatory protein p53, bcl? 2 expression. [Results] hyaluronan oligosaccharides can increase MCF? 7/ADM sensitivity to chemotherapy drugs, so that P? Gp, MRP decreased expression, p53 expression increased. [Conclusion] hyaluronic acid oligosaccharides can be partially reversed MCF? 7/ADM resistance and to promote apoptosis. [Keywords:] hyaluronic acid oligosaccharides MCF? 7/ADM reversal of apoptosis resistance Tumor cells to chemotherapeutic drugs is a multi-drug resistant tumors of the important reasons for treatment failure, to overcome multidrug resistance in one of the ways is to use reversal agents [1,2]. Despite the reversal of the current multi-drug resistance found in many drugs, however, not satisfied or because the effect, or their toxic side effects, it is difficult in clinical practice. In this study, the use of adriamycin-resistant human breast cancer cell lines in order to MTT method, flow cytometry, research hyaluronic acid oligosaccharides (hyaluronate oligomers, oligo? HA) on the sensitization-resistant cells and drug resistance reversal effect, and by lowering the surface membrane transporter protein expression and promote apoptosis resistance point of view to explore the mechanism of reversal of drug resistance in order to be able to find the role reversal of MDR strong, and toxicity of drugs. 1 Materials and methods 1.1 Materials Breast cancer MCF? 7 / S-sensitive cells and adriamycin-resistant MCF? 7/ADM cells, derived from Chinese Academy of Medi