HVEGF165 gene transfected bone marrow mesenchymal stem cell transplantation for myocardial infarction in rabbits.docVIP

 PAGE \* MERGEFORMAT 4 HVEGF165 gene transfected bone marrow mesenchymal stem cell transplantation for myocardial infarction in rabbits Authors: Sheng Xiaogang, SONG Hui, Feng Zhang, Chen Qiuxiong, Wu Shu-lin [Keywords:] myocardial infarction;, bone marrow mesenchymal stem cells;, vascular endothelial growth factor [Keywords:] myocardial infarction; bone marrow mesenchymal stem cells; vascular endothelial growth factor 1 Materials and methods 1.1 Materials Healthy purebred New Zealand white rabbits 48, male and female informal, body mass of 2.0 ~ 2.5 kg, rabbit age 4 ~ 5 mo, the Lingnan University and Research Animal Center. Sugar DMEM (Gibco products); Percoll separation medium (Pharmacia product); VIII factor polyclonal antibody and immunohistochemistry kit (Wuhan Boster Biological Engineering Co., Ltd.); BrdU mAb BrdU Detection Kit (Roche Products); AdTrackCMVVEGF165 eukaryotic expression plasmid (from the Guangdong Provincial People’s Hospital Central Laboratory as gifts); LipofectamineTM 2000 reagent (GIBCOL). 1.2 Methods 1.2.1 Experimental Group The rabbits were randomly divided into 4 groups (n = 12), myocardial infarction 2 wk after myocardial infarction in the infarcted group (MI group) injected with normal saline; MSCs transplantation group (MSC group) injection of 107 MSCs; VEGF group was injected with VEGF gene ( 100 μ g dissolved in 200 μ L PBS in); MSCs VEGF group (MV group) injection of 107 transfected with VEGF gene in MSCs. in the 3 d before transplantation to VEGF gene transfer. 1.2.2 Establishment of rabbit model of myocardial infarction in the left anterior descending artery ligation of the middle of production of myocardial infarction model. 1.2.3 rabbit bone marrow stem cells isolated and cultured in the proximal tibia bone marrow blood is about 4 ~ 6 mL, application of Percoll separation medium (1.073 kg / L) centrifugal extraction MSCs, containing 100 g / L FBS DMEM for low-sugar culture. near the confluence of the cells when