In patients with chronic HCV infection in PBMC in vitro secretion of Th1 type cytokines and related factors.docVIP

 PAGE \* MERGEFORMAT 5 In patients with chronic HCV infection in PBMC in vitro secretion of Th1 type cytokines and related factors Authors: Li Yue Qi, Xu Penghui, the Soviet Union waterfront, Chi Shu-Ping, Zhu Lei, Qi Yang, LI Jin-bo, Cheng Yun [Abstract] Objective: To study the chronic HCV (hepatitis C virus) infection in patients with peripheral blood mononuclear cells (PBMC) in vitro secretion of Th1 type cytokines, a better understanding of chronic HCV infection and liver injury. Methods: Chronic HCV infection in vitro patient PBMC 72 h after, ELISA testing supernatants of Th1 type cytokines (IL  2, IFN  γ , TNF  α ), fluorescence quantitative PCR detection of serum HCV RNA level, RT  PCR detection of HCV RNA subtype. Results: Compared with normal, IFN  γ in HCV RNA-negative patients was significantly higher in the RNA-positive patients was significantly higher in the no. TNF  α , regardless of the level of RNA titers were significantly higher than those of normal people. RNA-positive patients, ALT (alanine aminotransferase), AST (aspartate aminotransferase) levels were significantly higher than negative patients. a different HCV genotype infection in patients with IFN  γ , TNF  α secretion was no significant difference. Conclusion: IFN  γ in the rid of the virus play an important role, TNF  α is an important factor in liver injury caused by one. [Keywords:] C-type hepatitis-like virus 0 Introduction HCV (hepatitis C virus) infection easy to chronic, but its chronic mechanism is not yet very clear. Some studies [1-2] showed that natural immunity, humoral immunity and cellular immunity have cleared the virus plays an important role, while the cell-mediated immunity is particularly important. Th1 type cytokines are involved in cell-mediated immunity of the important material in the cell depletion of intracellular pathogens during the immune response play a key role, but is also an important mechanism induced liver injury. we compa