In rats nuciferine absolute bioavailability of.docVIP

 PAGE \* MERGEFORMAT 9 In rats nuciferine absolute bioavailability of Study: Water Xie Lin, Wang Yi Yao, Tan Yuxi, Lupei Feng, Tian Chunjuan, wearing PeopleSoft [Abstract] Objective To study in rats nuciferine pharmacokinetic profile and absolute bioavailability. Methods SD rats were intravenously (5 mg kg-1) and oral (20 mg kg-1) Netherlands leaf base, at different time points after administration of blood, separated plasma, LC MS / MS method for the determination of drug plasma concentration of lotus leaf base with DAS software to process data and calculate the pharmacokinetic parameters, find the absolute bioavailability. Results lotus leaf base intravenous pharmacokinetic parameters: AUC (0 - ) as (1 118.24 + -420.90) ng h mL-1, Cmax was (1 213.17 + -359.29) ng mL-1, t1 / 2 (1.30 + -0.69) h; oral nuciferine the pharmacokinetic parameters: AUC (0 - ) to (3 111.34 + -1 986.29) ng h mL-1, Cmax were (1 257.50 + - 942.37) ng mL-1, t1 / 2 was (4.89 + -2.88) h, tmax is (0.33 + -0.18) h; dose normalization calculated in rats nuciferine absolute bioavailability was 69.56 %. Conclusion The method is specific, sensitive and can be used for quantitative analysis of in vivo nuciferine. [Keywords:] nuciferine; pharmacokinetics; absolute bioavailability; LC MS / MS method Abstract: Objective To investigate the pharmacokinetic characteristics of nuciferine and its absolute bioavailability in rats.Methods Iv (5 mg kg-1) and po (20 mg kg-1) administration were given to SD rats respectively, and the plasma concentration of nuciferine in rats was determined by LC MS / MS.Results The main pharmacokinetic parameters through iv and po were as followed: respectively, AUC (0 - ) (1 118.24 + -420.90) and (3 111.34 + -1986.29) ng h mL-1, Cmax (1 213.17 + -359.29) and (1 257.50 + -942.37) ng mL-1; t1 / 2 (1.30 + -0.69) and (4.89 + -2.88) h. tmax was (0.33 + -0.18) h via po. The absolute bioavailability was 69.56% after dosage correction.Conclusion This method