Isolation and identification of solid tumor stem cells Research Progress.docVIP

 PAGE \* MERGEFORMAT 26 Isolation and identification of solid tumor stem cells Research Progress [Keywords:] cancer stem cell isolation and identification technology and methods Cancer stem cells (Cancer Stem Cell, CSC is the cancer research hot spots. According to cancer stem cells point of view, the tumor cells was heterogeneous, a few of the CSC decided to tumor formation, recurrence and metastasis, this is the traditional cancer treatment forming a large impact, so the issue is currently controversial .1997 for the first time isolated cancer stem cells - the blood system in acute myeloid leukemia stem cells, followed by solid tumors in cancer stem cell research have gradually carried out, from human brain tumors , breast cancer, liver cancer, kidney cancer and other cancer patients and cell lines successfully separated, identified cancer stem cells. visible to figure out the nature of CSC, the first task is to tumor tissues and cell lines CSC Purification and identified. all kinds of literature are combined with isolation and identification methods are summarized below in order to better study the CSC. A cancer stem cell separation method CSC in the current literature the main methods for separating cancer stem cells using cell surface markers (cell-surface marker sorting, and biological characteristics according to their functional separation into two categories. 1.1 The use of cell surface markers separating the current discovery of cancer stem cells and more is the use of marker separation found. Solid tumors CSC markers and the isolation and identification methods are using a normal stem cell research, stem cells of the rigorous identification and separation is only in very few organizations in the completion of many entities of the organization itself stem cell surface markers has not been determined. so the CSC phenotype of isolation and identification is also limited to a small number of solid tumors. isolated with this marker of cell