Jiangsu Glechomae HPLC fingerprint of.docVIP

 PAGE \* MERGEFORMAT 13 Jiangsu Glechomae HPLC fingerprint of Author: Zhu Ling-ying, Qian Wei, DUAN Jin-Ao, Qian Shi-Hui [Abstract] Objective To study the establishment of high performance liquid chromatography Glechomae (HPLC) fingerprint analysis method for quality control and provide the basis for identification of medicinal materials. Methods using high-performance liquid chromatography using reversed-phase Symmetry C18 column (4.6 mm * 250 mm, 5 μ m); mobile phase of acetonitrile and 0.4% phosphoric acid solution (gradient elution); detection wavelength 327 nm; column temperature 30 ℃ ; flow rate of 1.0 ml / min. Injection volume 20 μ l. The results in the selected chromatographic conditions, determine the 18 peaks with the money constitutes a fingerprint characteristics of herbal materials, each batch of herbs all have these characteristics, but the characteristic peaks of the relative content of chromatographic profile of the distribution of differences in cause there are some differences. Established HPLC-Glechomae of chlorogenic acid, flavonoids luteolin 2 retention time corresponding to the same; 10 batches Glechomae HPLC-fingerprint similarity were greater than 0.93. Conclusion The method is accurate, simple, stable, and its chromatographic fingerprint can be used for even money, identification and quality control of grass. [Keywords:] Abstract: ObjectiveTo establish an HPLC fingerprint of Herba Glechomae.MethodsThe HPLC method was used, and the chromatography conditions were Symmetry C18 column (250 mm * 4.6 mm, 5μ m) with mobile phase of acetonitrile - 0.4% phosphoric acid dihydrogen phosphate solution of water; the UV detection wavelength was 327 nm and column temperature was 30 ℃ with the flow rate of 1.0 ml / min; the injection volume was 20μ l. ResultsIn this chromatography condition, eighteen peaks were identified as the characteristic fingerprints of Herba Glechomae . All samples tested contained the same eighteen peaks, but the content o