LHR eukaryotic expression vector and expression.docVIP

 PAGE \* MERGEFORMAT 9 LHR eukaryotic expression vector and expression [Abstract] Objective To construct the luteinizing hormone receptor (LHR eukaryotic expression vector and the expression of LHR and establish a stable breast cancer cell lines. Methods Kpn / Hpa digested LHR cDNA plasmid vector to obtain the target gene cloned construct pcDNA3.1 (+) eukaryotic expression vector, restriction map and sequence analysis, recombinant plasmid by the liposome MCF 7 breast cancer cells, G418 selection, RT PCR, determination of intracellular cAMP, Screening and identification of high expression of LHR MCF 7 cells . Results The recombinant plasmid pcDNA3.1 (+) LHR restriction map analysis, sequence analysis showed that pcDNA3.1 (+) LHR LHR vector sequence of the LHR gene sequences with GenBank exact match. RT PCR detection of MCF 7 cells LHR mRNA, MCF cAMP concentrations were measured in 7 cells, confirming expression of MCF 7 cells LHR. Conclusion constructed and transfected pcDNA3.1 (+) LHR eukaryotic expression vector, in MCF 7 cells, stable expression, to explore the role of hCG on breast cancer cells to provide experimental basis. [Keywords:] Receptors, LH, human chorionic gonadotropin, breast cancer, cloning cells, genetic vectors, gene expression RNA, Messenger Human chorionic gonadotropin (human chorionic gonadotropin, hCG) is secreted by trophoblast cells have important physiological functions of a class of glycoprotein hormones with luteinizing hormone receptor (luteinizing hormone / human chorionic gonadotropin receptor, LHR to play with biological effect [1]. recent study found that hCG receptors in breast cells exist, so some scholars think that the breast cells during pregnancy hCG directly after giving birth may lower the incidence of breast cancer in women related to [24]. the author through the creation of high LHR expression in breast cancer cell lines, of hCG direct effect on breast cancer cells to provide experimental cell model. 1 Mat