Limulus anti-lipopolysaccharide factor on the late inflammatory factors in the high mobility group box protein1 in vitro effect of antagonism.docVIP

 PAGE \* MERGEFORMAT 6 Limulus anti-lipopolysaccharide factor on the late inflammatory factors in the high mobility group box protein1 in vitro effect of antagonism Author: Wu Lin Qing, Xu Wei Qun, Zhang Qiuyu, Hu Haixia, Zhu advanced, SU Dong-hui [Abstract] Objective To observe the Limulus anti-lipopolysaccharide factor (LALF) late on murine macrophage inflammatory factor high mobility group box protein  1 (HMGB1) functions antagonistic effects of endotoxemia LALF for advanced animal protection mechanisms. Methods HMGB1 stimulated mouse peritoneal macrophages, to join or not LALF, migration experiments testing the role of macrophage chemotaxis, ELISA detection of supernatant of tumor necrosis factor (TNF)  α concentration. The results of macrophage migration index showed an increase in HMGB1 dose-dependent, HMGB1 stimulated macrophage TNF  α concentration in supernatant were significantly higher. LALF pre-mixed with HMGB1 significantly decreased macrophage migration index and the TNF  α concentration in culture supernatants. Conclusion LALF by inhibiting HMGB1 may exert its protective effect of late septic animals. [Keywords:] high mobility group protein; sepsis; Limulus anti-lipopolysaccharide factor; chemotaxis ABSTRACT: Objective To investigate the effects of limulus anti  lipopolysaccharide factor (LALF) on the late proinflammatory cytokine of high mobility group box 1 (HMGB1). Methods The primary mouse macrophages were cultured and stimulated by HMGB1. Macrophage migration was determined by the use of chemotaxis chamber. ELISA quantitated the culture supernatants for TNF  α production. LALF was added to explore the effects of HMGBI and TNF  α production in culture. Results The chemotactic index of macrophages and the concentration of TNF  α in the culture supernatants evidently increased in dose  dependent manner in HMGB1 groups and prominently decreased by the LALF co  incubated. Conclusion HMGB1 was firstly indicated in t