Load DC-induced mannose glycosylated antigen specificity of anti.docVIP

 PAGE \* MERGEFORMAT 9 Load DC-induced mannose glycosylated antigen specificity of anti Author: Zhong Cheng, Xu, Ming Shi, Hu Mei-Ru, Min-Min, Chen Jian [Keywords:] dendritic cell;, glycosylated antigen;, tumor immunity Specific cytotoxicity of CTL which was induced by DC pulsed with mannosylated antigen [Abstract] AIM: To investigate the role of mannosylated tumor antigen in the course of antitumor response. METHODS: L2 domain of HER2/neu (ErbB2) ectodomain was expressed in E.coli, purified and mannosylated. Dendritic cells (DCs) were induced from human peripheral blood mononuclear cell by GMCSF and IL4. The maturation and functional capacity of DCs pulsed with mannosylated L2 (mL2) protein was investigated. RESULTS: L2 protein could induce DC maturation, which was accompanied by elevated expression of MHC and costimulatory molecules. The effect of mL2 protein on DC maturation was more remarkable than that of nonmannosylated L2 protein. Furthermore, DCs pulsed with mL2 could stimulate high tumor cell lysis by CTL. CONCLUSION: Our experiment provide the foundation for the study of new tumor vaccine by mannosylation. [Keywords] dendritic cells; mannosylated antigen; tumor immunity [Abstract] Objective: To explore the mannose glycosylated tumor vaccine in the role of anti-tumor immunity. Methods: The purpose of purified protein HER2/neu extracellular ligand first two domains (RLD2), and Glycosylation them, since the peripheral blood mononuclear cells induced by CD83 of the DC, the use of DC surface mannose receptor , so that the load DC The purpose of mannose glycosylated antigen mRLD2 (mL2), and sensitized T lymphocytes, CTL-specific anti-SKBR3 cells observed effect. Results: Compared with Glycosylation does not carry out the purpose of protein RLD2 (L2), mL2 can promote DC maturation and can induce a stronger anti-effectiveness of CTL. Conclusion: The results of this experiment to carry out a new mannose glycosylated tumor vaccine provides a theoretical b