4株乳酸杆菌体外拮抗3种常见致病菌的研究.pdfVIP

安徽农业科学， Joumal of Anhui A伊. Sci. 2013 ,41 (8) :3314 -3315 ,3326 责任编辑朱琼琼责任校对卢篇 4 株乳酸杆菌体外拮抗 3 种常见致病菌的研究 杨小进，张华菲，何希宏，刘浩* (天津科技大学生物工程学院，工业发酵微生物教育部重点实验室，天津 3∞457) 摘要 [目的]研究 4 株乳酸杆菌在体外对 3 种常见致病菌的拮抗作用。[方法]将 4 株乳酸杆菌(L1、 12 、L3、lA)分别与 3 种致病菌(大 肠杆菌、白色念珠菌和金黄色葡萄球菌)共培养，观察拮抗结果。[结果] 4 株乳酸杆菌对 3 种致病菌都有显著的拮抗作用，其中L1的拮 抗作用强于其他 3 株乳酸杆菌;当将致病菌的菌落数提高一个数量级时，L1仍可将 3 种致病菌杀死，拮抗作用仍十分显著。[结论]该研 究为今后用乳酸杆菌直接进行微生态治疗提供了一定的理论基础。 关键词乳酸杆菌;致病菌;拮抗作用 申图分类号 5188 文献标识码 A 文章编号 0517 -6611 (2013 )08 -03314- 但 Study on Antagonism of Four Lactobacillus Strains on Three Common Pathogens in viTη YANG Xiao-jin et a1 (Key Laboratory of Industrial Fennentation Microbiology of Ministry of Education , College of Biotechnology ,Tianjin Uni? versity of Science Ik Technology ,Tianjin 3α457) Abstract [ Objective] The paper w副 to study the antagonism of fo町 lactobacillus strains on three common pathogens in vitro. [Method] Four lactobacillus strains (L1 ，口，口，lA) were cuItured with three pathogens (Escher?chia coli ,Candida albica阳，5;ωtphylococCWi α町e旧) respecti vel y , and the a阳在:onistic resuI恼 were observed. [ResuIt] Four lactobacillus strains had significant antagonism on 也ree pathogens , and the antagonism of L1 w幽幽unger than the other three strains; when the number of colonies w由 increased by one order of magnitude ,L1 couId still kill the three pathogens ,and the antagonism w臼 still ve巧 significant. [ Conclusion] 币le study provided a certain theoretical basis for the future use of lactoba? cillus directly in microecologics treatment. Key words Lactobacillus; Pathogens; Antagonism 乳酸杆菌是一类(属)能分解多种糖类产生大量乳酸的