Mesenchymal stem cells in culture and settle in the ability of the liver transplant study.docVIP

 PAGE \* MERGEFORMAT 14 Mesenchymal stem cells in culture and settle in the ability of the liver transplant study [Abstract] Objective isolated and identified in vitro amplification of rat bone marrow mesenchymal stem cells (MSC), MSC in liver transplant recipients observed in vivo settlement capabilities. Method directly adherent cultured rat MSC, the cell growth curve; flow cytometry to detect cell surface markers. Induction of MSC into osteoblasts and adipocytes differentiation, identification of their multiple differentiation potential. DAPI fluorescence MSC, from the portal vein into the liver transplantation, frozen sections of liver tissue was observed in the transplanted liver settlement. Results were successfully directly isolated adherent MSC, and can be subcultured in the purified and amplified. passaged period of about 5 d, In vitro generation of in more than 20 generations, with a strong proliferation. consistent with flow cytometry MSC phenotype. MSC can be successfully differentiated into bone cells and fat cells, multipotent differentiation capacity. DAPI positive marker was 100% MSC. fluorescence microscope, can be seen located in the graft liver MSC. Conclusions The direct adherence MSC isolation and culture of easy to obtain MSC high purity, biological characteristics and stability; MSC can survive and settle in the liver transplant. [Keywords:] were isolated and cultured; identification; rat; liver transplantation Mesenchymal stem cells (mesenchymal stem cells, MSC) is an adult stem cell, with a high degree of proliferation and differentiation potential. Domestic and foreign scholars MSC in Acute myocardial infarction, cerebral infarction, liver failure, extensive burns organizations such as wound healing, and has achieved significant results. MSC has a low immunogenicity, autologous or allogeneic Zhiru no significant rejection, in vitro transfection efficiency and high, is genetic engineering a good carrier. The author of the MSC w