Metabolism in patients with liver cirrhosis group H NMR study.doc

 PAGE \* MERGEFORMAT 11 Metabolism in patients with liver cirrhosis group H NMR study Of: Yangyong Xia, Yang Shengyi, Liangmin Feng, Qiu Cuihuan, Zhang Wei, Xu Jingfen [Abstract] Objective H NMR (1H nuclear magnetic resonance, 1H NMR) and principal component analysis (principal component analysis, PCA) method cirrhosis group of patients with metabolic changes. Methods 20 patients with chronic hepatitis B patients and patients with liver cirrhosis in 18 cases, using BRUKER Avance 500 MHz superconducting NMR spectrometer were detected and analyzed using pattern recognition methods in patients with liver cirrhosis serum metabolomics. Results of hepatitis B patients and patients with serum hepatitis B cirrhosis metabolic group has significant difference. cirrhosis of the liver caused by the serum lipid and choline / phosphocholine decreased, while lactic acid, alanine, methionine, glutamine, creatine and glucose content increased. Conclusion Shangshu metabolite changes can distinguish between patients with hepatitis B and liver cirrhosis. Qing-based magnetic resonance spectroscopy and principal component analysis, metabolomics method can provide a reliable diagnosis of cirrhosis at the molecular level of the metabolic basis. [Keywords:] Magnetic resonance NMR; cirrhosis; metabolism; principal component analysis Abstract: Objective 1H Nuclear magnetic resonance (1H NMR) spectroscopy in conjunction with principal component analysis (PCA) had been employed to characterize the metabolite composition of the serum samples from the patients with posthepatitic cirrhosis.Methods 1H NMR spectra were measured for serum samples from 20 patients with chronic hepatitis B and 18 patients with posthepatitic cirrhosis, and the spectra data were analyzed by PCA to reveal the metabonomic changes.Results There were clear metabonomic differences between the patients with chronic hepatitis B and the patients with posthepatitic cirrhosis that could lead to decreased levels in