Metallothionein MT2a Cd and Cu complexation with electrospray ionization mass spectrometry characterization.docVIP

 PAGE \* MERGEFORMAT 17 Metallothionein MT2a Cd and Cu complexation with electrospray ionization mass spectrometry characterization Of: Chen Jincan Yewei Xiang Kang Haining Ge Liya Wang Zhuang Zhi House as [Abstract] Electrospray ionization mass spectrometry (ESI MS study of metallothionein (MT, there are two domain @ and β 2a and the metal ions Cd  and Cu  the complexation. MT 2a purified by a reverse phase chromatography system too, and electrospray mass spectrometry identification. by ESI MS MT 2a study with different amounts of Cd and Cu in the complex, the results indicate that, Cd  to give priority to the @ domain with the MT complex, the formation of M2 +4 S11 structure, and The complex process of the obvious synergies, Cd  and the β domain of MT complex in the form of M2 +4 S9, it presents the obvious characteristics of the random loose complex, Cu  priority and the β domain of MT complex , and its complexation by the Cu4 gradual transition to a higher bound, with higher levels in the Cu  conditions, Cu  and MT showing a variety of complex ways. [Keywords:] ionization mass spectrometry, metallothionein, cadmium ion , copper ion , complexation Abstract Mammal metallothionein (MT) folds into two separate domains that exhibit different structure and metal binding propertity independently, the study of the strategy of metal ions binding with MT would give better understanding of their exact biological functional mechanisms. In this study, a method using eletrospray ionization mass spectrometry (ESI MS) had been developed and employed to study the stoichiometries of Cd  and Cu  binding with MT 2a. MT 2a was purified from MT mixture by reversed phase liquid chromatography and identified by ESI MS. Different amounts of Cd or Cu were then added in MT 2a samples and ESI MS was employed to determine the mass difference of MT in different samples. The results showed that Cd  prefer to cooperatively bind in @ domain with the form of Cd2 +4 S