Microarray - comparative genomic hybridization technologies and their application in cancer research.docVIP

 PAGE \* MERGEFORMAT 33 Microarray - comparative genomic hybridization technologies and their application in cancer research Abstract Microarray comparative genomic hybridization (array-CGH) technology is the DNA cloning or cDNAs made micro-array, instead of the traditional CGH method in metaphase chromosomes as hybridization targets, not only to increase the resolution, or even determine the tumor-related gene and to provide accurate positioning. Array-CGH full overview of the principles, methods and its application in oncology and significance. Keywords: micro-arrays - Comparative genomics Microarray comparative genomic hybridization (array-comparative genomic hybridization, Array-CGH) technology is the DNA cloning or cDNAs made of micro-array, instead of the traditional CGH detection of metaphase chromosomes as hybridization targets will be tested, not only to increase the resolution but also to determine the tumor-associated genes and provide accurate positioning. At the same time can be identified by the computer software of each chromosome, to overcome the need to identify the chromosome of experienced personnel constraints, for the rapid and comprehensive analysis of tumor DNA copy number changes, and detection of chromosomal instability provides a more desirable method. This article on the technical principles, methods and their application in cancer research to make a brief overview. A micro-array - an overview of comparative genomic hybridization 1.1 Array-CGH basic principle of Array-CGH and CGH similar, but it is used or cDNAs cloned DNA microarray chip shop instead of metaphase chromosomes as hybridization targets, about the same amount of different fluorescent markers under test and reference DNA-to-human Cot-1DNA closure of non-specific repeat sequence After hybridization to the same time, or the cDNAs cloned by the DNA composition of micro-array. Using micro-array for each target on the two kinds of si