Microbial sources of antibiotic JXJ-301-1 Study.docVIP

 PAGE \* MERGEFORMAT 8 Microbial sources of antibiotic JXJ-301-1 Study Author: Zhang Bing-fire Lee Han had seven full-Zhang YQ Yang Yuan-wen Meng-Liang [Abstract] from an unidentified strain of the secondary metabolites of microorganisms isolated a compound JXJ-301-1, through the generation of the compounds in deuterated chloroform and deuterated dimethyl sulfoxide two kinds of solvents in the NMR data analysis, combined with mass spectrometry, infrared spectroscopy and elemental analysis proved that the compound is podophyllotoxin. This study extends the source of podophyllotoxin. [Keywords:] micro-organisms; podophyllotoxin; nuclear magnetic resonance ABSTRACT A compound JXJ-301-1 was isolated from the fermentation broth of an uncharacterized microbial strain. Based on NMR, MS, IR and elemental analysis, its chemical structure was elucidated as podophyllotoxin originally produced by plants. This study extended source of podophyllotoxin and provided new way to produce podophyllotoxin by fermentation of microorganisms. KEY WORDS Microorganism; Podophyllotoxin; Nuclear magnetic resonance (NMR) Podophyllotoxin is a kind of anti-tumor activity of lignan natural products, mainly derived from the mandrake plants, can be used as semi-synthetic anticancer drug etoposide, teniposide, and etopophose precursors such as [1]. Due to a lack of natural sources of podophyllotoxin, while the synthesis is not yet achieved commercial requirements [2], thus limiting the further development of the drugs. Although studies have shown that the current mandrake plants can produce an endophytic fungus of podophyllotoxin [3], but rarely isolated from the microbial metabolites purified podophyllotoxin reported. In an unidentified microbial secondary metabolites course of the study, isolated to a compound JXJ-301-1, was identified as podophyllotoxin (chemical structure, see Fig.1). This paper reports the fermentation of the strain, compound JXJ-301-1 Purification and structural anal