Microfluidic Chip Electrophoresis in the separation of urine protein.docVIP

 PAGE \* MERGEFORMAT 15 Microfluidic Chip Electrophoresis in the separation of urine protein Author: Chu Shao-peng, WANG Hui-min, Wei Song, Cong-Hui, Wang Yueguo, Zhang Qin, Zhang Zhiquan, Xuyao Zhong, Qing-Hui Jin [Abstract] Objective: The microfluidic chip separation of urinary protein electrophoresis for clinical validation of tests to evaluate its clinical application value. Methods: The microfluidic chip electrophoresis on 30 cases of urinary protein qualitative test for the negative control group and 70 cases of urinary protein characterization in the above cases of group tests to white globulin peak area ratio to determine the selectivity of proteinuria and with United States Helena Comparison of agarose gel electrophoresis. Results: 30 cases of protein peaks were not detected in the control group, 70 cases Group, except in exceptional two protein peaks were detected, of which 16 patients with selective proteinuria, 50 cases of non-selective proteinuria, overflow proteinuria in 2 cases, and the United States Helena agarose gel electrophoresis results of 100% consistent with the clinical diagnosis consistent with the rate of 97.14%. Conclusion: The method used to separate the validation of clinical urine protein testing, worked well, suited for the general promotion of small and medium hospitals. [Keywords:] Microfluidic chips; electrophoresis; urinary protein; selective proteinuria [Abstract] Objective: Micro fluidic chip electrophoresis was used for verifying test of separating clinical urine proteins and was evaluated in clinic. Methods: Thirty urine samples whose qualitative tests of urinary proteins were negative and seventy urine samples whose qualitative tests of urinary proteins were more than were detected and the selectivity of proteinuria was judged by peak area ratio of albumin and globulin. The results were compared with American Helena agarose gel electrophoresis. Results: No protein peak was detected in thirty urine samples of the contr