Microwave-assisted extraction soil pachymaran.doc

 PAGE \* MERGEFORMAT 27 Microwave-assisted extraction soil pachymaran Author: Huang Shao-Wei CHI Ru-an Zhang Yue non - 【Abstract】 Objective To screen the soil pachymaran optimized extraction process. Methods of microwave-assisted extraction technology, through a single factor and orthogonal experiment to earth pachymaran extraction rate as an indicator to screen soil pachymaran the best extraction conditions. The results of soil Pachymaran optimum extraction conditions were: microwave power 800W, solid-liquid ratio (g / ml) 1:30, extraction temperature 100 ℃, reflux extraction twice, 10 min / times, concentrated liquid plus 90% ethanol alcohol analysis. Conclusion optimized process is stable, microwave-assisted extraction soil pachymaran, extraction time is short, high extraction rate, while a greater proportion of total flavonoids from Smilax glabra Roxb also been leaching. The optimized process conditions in accordance with experiment, soil pachymaran extraction rate, the yield of crude polysaccharide and polysaccharide in the polysaccharide content were 8.33%, 3.97%, and 39.5%. Keywords: microwave-assisted extraction of total flavonoids soil pachymaran Abstract: ObjectiveTo optimize the process of extracting polysaccharides from Smilax glabra Roxb. MethodsWith the extraction rate of polysaccharides as an examinable target, the optimal extraction conditions of polysaccharides has been proposed by microwave-assisted method through single factor experiment and orthogonal test method. ResultsThe optimal technological parameters of extracting polysaccharides were as follows: the microwave power, the solid-liquid ratio, the extract temperature and extract time were 800 W, 1:30 (g / ml), 100 ℃ and 10min twice, respectively. Lastly, the crude polysaccharides were obtained by adding ethyl alcohol into the treated leachate after laying a period.ConclusionThe proposed method is stable and reliable for the extraction of polysaccharides from S