mig14 typhoid Salmonella gene expression in hyperosmotic stress in the regulation of early.docVIP

 PAGE \* MERGEFORMAT 16 mig14 typhoid Salmonella gene expression in hyperosmotic stress in the regulation of early Study: Summer Autumn, Zou Xin, Du Hong, high-Yu Lin, Huang Xinxiang [Abstract] Objective: To detect Salmonella typhi mig 14 under hyperosmotic stress in the regulation of the expression of certain genes. Methods: The suicide plasmid-mediated homologous recombination method of preparation of Salmonella typhi mig 14 gene deficient mutant strain, using genes microarray analysis of early hyperosmotic stress and mig 14 wild strains deficient strain differences in gene expression, and some results using real time quantitative PCR validation. Results: The preparation of Salmonella typhi strains defective mig 14, mig 14 variant Early hyperosmotic stress compared with the wild type strains of 77 genes and 72 genes were down regulated. Conclusion: mig 14 may be a regulatory gene, mainly involved in the regulation of substances and energy metabolism of bacteria. [Keywords:] Salmonella typhi, mig 14, hypertonic stress, microarray analysis [Abstract] Objective: To explore the function of mig 14 of Salmonella enterica serovar Typhi in response to hyperosmotic stress.Methods: A mig 14 gene deleted mutant of S.enterica serovar Typhi was constructed using the homologous recombination method by means of a suicide plasmid ; the difference of the gene expression between the wild strain and the mig 14 mutant at early stage of hyperosmotic stress was investigated by genomic microarray assay and partially confirmed by real time quantity reverse transcription PCR. Results: Successfully constructed a mig 14 mutant; about 77 and 72 genes were down and up regulated respectively at early stage of hyperosmotic stress in mig 14 mutant. Conclusion: mig 14 may be a regulator, may primally regulate the substance metabolism and energy metabolism of Salmonella enterica. [Keywords:] Salmonella enterica serovar Typhi; mig 14; hyperosmotic stress; DNA microarrays Prok