Mitochondrial DNA D loop in Yunnan Nu HVRgenetic polymorphism.doc

 PAGE \* MERGEFORMAT 10 Mitochondrial DNA D loop in Yunnan Nu HVRgenetic polymorphism Study: High Shu Fai, Guihong Sheng, Sheng-Bin Li [Abstract] Objective To observe the Yunnan Nu population of mitochondrial DNA D loop hypervariable region (HVR ) single nucleotide polymorphisms (single nucleotide polymorphism, SNP) of the population genetic characteristics, in order to provide forensic DNA sequence polymorphism of the mitochondrial basic data. Methods ABI3730 Genetic Analyzer, PCR product direct sequencing method, Yunnan, China Nu 65 unrelated individuals of blood was extracted using chelex 100 mitochondrial DNA D loop hypervariable region sequence . Results of mitochondrial DNA in high variable region (HVR ) 73 ~ 366 with Anderson sequence comparison between the Yunnan Nu total of 65 unrelated individuals, 59 haplotypes, 401 mutations. SNP degree of genetic difference 0.9957, nucleotide polymorphism is 0.1174. Conclusion Yunnan Nu mitochondrial DNA D loop region of HVR SNP with a high degree of polymorphism, is a group of individuals Nu identification, paternity testing, and national origin of genetic markers useful for molecular studies. [Keywords:] Nu HVR groups individual identification of mitochondrial single nucleotide polymorphisms ABSTRACT: Objective To investigate the mitochondrial DNA D loop region HVR single nucleotide polymorphism (SNP) of Nu ethnic population from Yunnan Province of China and to provide basic database of mtDNA sequence polymorphism for forensic identification purpose. Methods Genomic DNA from the whole blood of 65 unrelated individuals from Nu ethnic population living in Yunnan Province of China was extracted by standard chelex 100.The sequence polymorphism was analyzed by PCR based assay with ABI 3730 Analyzer to detect the number of relatively common point mutations. Results We compared the mtDNA HVR 73 ~ 366 with the Anderson sequence; 59 SNP loci were observed among them with 401 point mutations identified in