Mixed Phoxim and methomyl on male reproductive toxicity of cells.doc

 PAGE \* MERGEFORMAT 12 Mixed Phoxim and methomyl on male reproductive toxicity of cells Of: Li Honghui, Liu Xiufang, Ma Xiao, Zhang Guoqi [Abstract] Objective To observe phoxim and methomyl on male reproductive cells of the joint toxic effects. Methods adult 64 male Kunming mice were randomly divided into two batches, each batch were randomly divided into 4 groups, each Section 8, namely: negative control group (fed the same volume of distilled water), phoxim group (1/80LD50, 25mg/kg body weight), methomyl group (1/80LD50, 0.125mg/kg body weight), poverty Viagra + phoxim more mixed group (1/80LD50 +1 / 80LD50, 0.125mg / kg +0.125 mg / kg). the first batch of experimental animals exposed to continuous oral 4d, day 1, after the exposure Day 5 were killed by cervical dislocation, the production of mice right testis testis micronucleus observed. The second batch of mice exposed by gavage consecutive 30d, day 1, were killed after the last exposure, measuring small Rat organ weight, sperm parameters (sperm count, viability and malformation rate), testicular germ cell apoptosis. Results phoxim, a single dose of methomyl to testicular germ cell micronucleus rate was increased, decreased sperm motility, sperm abnormality rate increased (P lt;0.05 or P lt;0.01). phoxim + methomyl caused testicular mixed germ cell micronucleus rate increased significantly lower sperm motility, sperm abnormality rate increased (P lt;0.05 or P lt;0.01 ), there is interaction. Conclusions Mixed Phoxim and methomyl on male reproductive system has combined toxic effects, so that testicular germ cell micronucleus rate increased, decreased sperm motility, abnormal sperm increased testicular germ cell apoptosis levels increase, showed an antagonistic effect; Phoxim and methomyl mixture may be by inducing apoptosis of germ cells and reproductive cells micronucleus increases, thereby affecting the quality of sperm. [Keywords:] phoxim; methomyl; joint action; germ cell toxicity Survey s