Mixture of ferulic acid and paeoniflorin effect on PC12 cell injury_0.doc

 PAGE \* MERGEFORMAT 11 Mixture of ferulic acid and paeoniflorin effect on PC12 cell injury Of: Ma Lijun, Miao Zhen flowers, MA Wei, Tian Jianying [Abstract] Objective To observe Chae Wei acid and paeoniflorin mixture of FeSO4 and H2O2-induced PC12 cell injury, to explore its mechanism. Methods role of FeSO4 and H2O2 method of free radicals induced by PC12 cell injury model. Inverted phase contrast microscope for cell morphology and measured by MTT colorimetric cell viability, thiobarbituric acid method for the determination of malondialdehyde (MDA), xanthine oxidase method of superoxide dismutase (SOD) activity. FeSO4 and H2O2 damage results were compared, ferulic acid and paeoniflorin in the mixture can improve the number of PC12 cell survival and reduce the MDA content and increased SOD activity. Conclusion concentration of 5 ~ 50 μmol / L of ferulic acid and the concentration of 1 ~ 10 μmol / L of the mixture of FeSO4 and paeoniflorin PC12 cell damage induced by H2O2 showed a protective effect, and its mechanism may be related to ferulic acid and paeoniflorin in scavenging free radicals and inhibiting lipid peroxidation. [Keywords:] ferulic acid, paeoniflorin, PC12 cells Abstract: Objective To observe the protective effects of the mixture of ferulic acid (AF) and paeoniflorin on PC12 cells injury by FeSO4/H2O2 in vitro, and explorer its mechanism. Methods The cell injury model was established with FeSO4/H2O2 for 24 h. The process of the growth and morphology of the cells were observed under phase contrast microscope in vitro. The survival rate of the cells was determined by MTT method. The content of MDA and SOD activity was measured respectively by thiobarbituric acid and xanthine oxidase method. Results Compared with FeSO4/H2O2, the mixture of ferulic acid (AF) and paeoniflorin enhanced the PC12 cells survival rate and viability, reduced MDA content and increased SOD activity. Conclusion The mixture of 5 ~ 50 μmol / L AF and 1 ~ 10 μmol / L pa