Mortierella sp Δ6 prokaryotic fatty acid desaturase gene expression and transgenic plant regeneration.doc

 PAGE \* MERGEFORMAT 28 Mortierella sp Δ6 prokaryotic fatty acid desaturase gene expression and transgenic plant regeneration Author: Guang-Ke Wang, Ming-Chun, Jin-Chuan Li, Lai-Jun Xing 【Abstract】 Objective To Agrobacterium-mediated method will be derived from Mortierella sp of Δ6  fatty acid desaturase (D6D) gene into soybeans to northeastern Jilin Suinong 10 and 47 were eventually obtained transgenic plants. Methods cotyledon node explants induced multiple shoot clumps and regenerated plants using kanamycin resistance screening medium continuous screening and PCR methods, DNA analysis and GC analysis of molecular hybridization detection of transgenic plants in the Δ6  fatty acid desaturase status of gene expression. Results After containing 50 mg / L kanamycin resistance screening medium continuous screening, access to a number of transgenic plants. PCR detection and DNA by molecular hybridization analysis, preliminary evidence Δ6  fatty acid desaturase genes have been imported and integrated into the soybean genome. Of genetically modified soybeans on behalf of T1-and T2 seed fatty acids in GC analysis, the results detected in soybean seeds Δ6  product of fatty acid desaturase genes GLA, prove that the gene has been expressed in soybean. Keywords: Δ6  fatty acid desaturase gene; soybean; Agrobacterium-mediated transformation; fatty acid GC analysis; transgenic plant 【Abstract】 Objective The Δ6  fatty acid desaturase genes from Mortitieralla isabellina were introduced into suinong10 and jilin47 via Agrobacterium infection method, some transgenic soybean were obtained. Methods Adentitious buds and regenerated plants were inducted from cotylendon nodes, Kanamycin resistant culture medium, PCR Southern blot and GC analysis detected condition of the Δ6  fatty acid desaturase genes of transgenic plants. Result Kanamycin resistant (Kmr) transgenic plants were obtained by screening in selective condition. PCR and Sout