Muscle-derived neurotrophic factor in rat peripheral nerve injury repair.doc

 PAGE \* MERGEFORMAT 7 Muscle-derived neurotrophic factor in rat peripheral nerve injury repair Authors: Zhang Lianbin, Liu Ying, the beginning of Xiangyang, Hubei Lingling, Wang Bo [Keywords:] peripheral nerve injury Musclederived neurotrophic factor used in repairing peripheral nerve injury in rats [Abstract] AIM: To observe the effect of musclederived neurotrophic factor (MDNF) in repairing peripheral nerve injury in rats. METHODS: MDNF was extracted from adult rat skeletal muscle, and 10 μ L MDNF (0.1 mg / L) and 10 μ L sodium solution were respectively injected into the injured sciatic nerve segments. Sodium solution group and normal rats were employed as controls. The morphologic observation and stereological measurement of regenerated nerve were evaluated by HRP retrograde tracing method and Nissls staining and trichrome staining techniques. RESULTS: Some regenerated fibers passed through the injected sciatic nerve segments in MDNF group. The morphologic observation and stereological measurement of nerve regeneration by MDNF injection were better than those in sodium solution group. CONCLUSION: MDNF can promote the regeneration of injured peripheral nerves. [Keywords] neurobiology; musclederived neurotrophic factor; peripheral nerve scathe; peripheral nerve regeneration; HRP; rat [Abstract] Objective: To observe the muscle-derived neurotrophic factor (MDNF) on rat peripheral nerve injury repair. Methods: From the adult rat skeletal muscle extract MDNF, take 10 μ L MDNF (0.1 mg / L) was injected into rats segment of sciatic nerve injury in the experimental group at the same time set up experimental control group (injected with normal saline) and normal control group. 20 d after tracer method using horseradish peroxidase (HRP), Nissl staining, special three-color staining method of the regenerated nerve were observed and metrology statistics. Results: Injection MDNF the Department of peripheral nerve injury regeneration of nerve fibers through; MDNF