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Multi-drug resistant Klebsiella pneumoniae of aminoglycoside resistance genes
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Multi-drug resistant Klebsiella pneumoniae of aminoglycoside resistance genes
Of: Daixue Hai, Wang Huajun, gold method Cheung, Chiu-US
[Abstract] Objective: To understand the multi-drug resistant Klebsiella pneumoniae in the presence of aminoglycoside modifying enzyme gene status. Methods: From January 2006 to October 2006 patients were isolated and screened 20 strains of multi-drug resistant pneumonia Klebsiella, broth microdilution assay sensitivity of 20 kinds of antimicrobial agents; PCR detection of aminoglycoside modifying enzyme genes in 15. Results: 20 strains of multidrug resistant Klebsiella pneumoniae big celebration to aminoglycosides vancomycin, tobramycin, netilmicin and amikacin resistance rates were 75%, 80%, 65% and 60%, aminoglycoside modifying enzymes aac (3) - gene positive 15 , aac (6 ‘) - b gene positive one, ant (3 “) - gene positive 16, aph (3’) - gene positive 3, ant (2”) - gene positive one. Conclusion : multi-drug resistant Klebsiella pneumoniae resistant to aminoglycosides is mainly due to aac (3) - , aac (6 ‘)-Ib, ant (3 “) - , aph (3’) - and ant (2 “) - 5 kinds of aminoglycoside modifying enzyme genes exist; detected from the Klebsiella pneumoniae aac (6 ‘)-Ib-Cr-type and aph (3’) - are domestic for the first time.
[Keywords:] Klebsiella pneumoniae; aminoglycoside modifying enzymes; multiple drug resistance
Abstract: Objective: To understand the existence of genes encoding aminoglycoside modifying enzyme in multi-resistant Klebsiella pneumoniae. Methods: Samples of 20 strains of multi-resistant Klebsiella pneumoniae collected from the patients from January 2006 to October 2006 were isolated and screened out. The sensitivity of 20 antibacterial agents by means of broth induction was detected, and genes of aminoglycoside modifying enzyme by way of polymerase chain reaction were detected. Results: The resistance rates of 20 strains of multi-resistant Klebsiella pneumoniae to aminoglycoside,
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