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Mycobacterium tuberculosis Recombinant Protein Preparation and antigenic analysis of
PAGE \* MERGEFORMAT 16
Mycobacterium tuberculosis Recombinant Protein Preparation and antigenic analysis of
Author: Su rights, YUE Qiao-Hong, Zhou Ping, Duan Yan, Yang Liu, Yang Junlan, Liu Yun, Hao Ke
[Keywords:] Mycobacterium tuberculosis; antigen; gene expression; ELISA
Preparation and antigenicity assay of recombinant proteins of Mycobacterium tuberculosis
[Abstract] AIM: To clone and express the Mr 16 * 103 and Mr 38 * 103 proteins of Mycobacterium tuberculosis in E. coli, and to characterize its antigenicity and specificity. METHODS: The Mr 16 * 103 and Mr 38 * 103 antigen genes were amplified from Mycobacterium tuberculosis genome DNA by polymerase chain reactions and cloned into pGEX4T2 expression vector after sequencing. BL21 strain of E.coli was transformed with the recombinant vectors and induced to express recombinant proteins. The proteins were purified by affinity column chromatography. The antigenicity and specificity of purified proteins were estimated by enzymelinked immunoabsorbant assay. RESULTS: The BL21 strains of E. coli with recombinant vectors showed 42% and 18% of Mr 16 * 103 (688 mg / L) and Mr 38 * 103 (217 mg / L) gene expressions after induction. The sensitivity of Mr 16 * 103 and Mr 38 * 103 proteins were 67.0% and 79.8%, specificity were 100% and 99%, accuracy were 84.0% and 89.7% respectively. CONCLUSION: The expressions and purifications of recombinant Mr 16 * 103 and Mr 38 * 103 antigens with good antigenicity and specificity facilitate their research and clinical application in the diagnosis of Mycobacterium tuberculosis.
[Keywords] Mycobacterium tuberculosis; antigen; gene expression; enzymelinked immunoabsorbant assay
[Abstract] Objective: Cloning and Expression of Mycobacterium tuberculosis Mr 16 * 103 and Mr 38 * 103 recombinant proteins were measured antigenicity and specificity. Methods: Using polymerase chain reaction from the genome of Mycobacterium tuberculosis DNA amplification Mr 16 * 103 and Mr 38 * 103 anti
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