Multiplex PCR Detection of IgVH gene mutations in chronic lymphocytic leukemia.doc

 PAGE \* MERGEFORMAT 4 Multiplex PCR Detection of IgVH gene mutations in chronic lymphocytic leukemia Author: ZHENG Wen-juan Chen Lijuan Wu Yu-Jie Li Li Xu Wei Li Jianyong [Abstract] immunoglobulin heavy chain variable region (IgVH) gene mutations of chronic lymphocytic leukemia (CLL) most important independent prognostic factor, in order to explore the IgVH gene mutation status of CLL patients, Multiplex PCR to detect nine cases of CLL patients with IgVH gene, purification of PCR amplified products by direct sequencing, the application IMGT / V-QUEST tool to analyze and clearly whether IgVH Mutation and location. The results showed that: nine cases of CLL patients were amplified 395-465 bp region (MIX I) or 290-360 bp region (MIX II) monoclonal bands, sequencing results showed that five cases of patients have a mutation, IgVH genes were IGHV3 - 11 * 03, IGHV3-9 * 01, IGHV3-23 * 01, IGHV4-59 * 01 and IGHV4-34 * 02; 4 cases without mutation, IgVH gene IGHV3-53 * 01, IGHV3-23 * 03, IGHV3 - 33 * 05 and IGHV3-7 * 01. Conclusion: PCR Detection of IgVH gene mutation, to simplify the tedious experimental process, shortening the experimental time to solve the traditional PCR detection of IgVH gene mutations in shackles worth promote the use of clinical and scientific research. [Keywords:] multiple PCR; IgVH; gene mutation Detection of IgVH Mutation Status in Patients with Chronic Lymphocytic Leukemia by Multiplex PCR Abstract IgVH mutation status is one of the most important independent prognostic factor of chronic lymphocytic leukemia (CLL). In order to evaluate IgVH mutation status in patients with CLL, IgVH mutation was detected by multiplex PCR in 9 CLL patients and purified PCR amplification products were directly sequenced, IgH somatic hypermutation and mutation site were analysed by IMGT / V-QUEST. The results showed that 5 patients had mutated IgVH, and IgVHs were IGHV3-11 * 03, IGHV3-9 * 01, IGHV3-23 * 01, IGHV4 -59 * 01, IGHV4-34 * 02, respectively; wh