Mulberry total alkaloids analysis method and extraction method of.doc

 PAGE \* MERGEFORMAT 4 Mulberry total alkaloids analysis method and extraction method of [Abstract] Objective To study the total alkaloids in mulberry analytical methods and extraction methods. Methods of physical and chemical reactions and thin-layer chromatography for qualitative and quantitative analysis of alkaloids in mulberry leaves. Mulberry alkaloids through the analysis of structural features of the design method of extraction and purification. The results developed thin-layer chromatography method of mulberry alkaloids are given from the mulberry leaf, mulberry, Morus alba L., and total alkaloids extracted mulberry stalk method. Conclusion TLC - visible spectrophotometry can be used for quantitative analysis of total alkaloids of Mulberry, the proposed method has a total alkaloid extract operability. [Keywords:] Mulberry; total alkaloids; analytical methods; extraction method Abstract: ObjectiveTo study the analysis and extraction methods for total alkaloids (MTA) in mulberry.MethodsIdentifying reactions and thin-layer chromatography (TLC) for qualitative and quantitative analysis were adopted, and molecular structural characteristics for extraction and purification scheme design were deduced. ResultsTLC method and extraction method of Folium Mori, Fructus Mori, Cortex Mori and Ramulus Mori were developed. ConclusionMTA can be determined by TLC-VIS spectrophotometry.The preparation process of MTA in this article is practicible. Keywords:: Mulberry; Total alkaloids; Analysis method; Extraction method Most of alkaloid components of mulberry DNJ (nojirimycin) derivatives. DNJ was first produced in 1965 and rose-color from the Streptomyces R-468 (Streptomyces roseochromogenes R-468) of the fermentation broth were isolated [1], the structure was identified in 1966 [2], 1967, has been synthesized 1,5 -- imino-1 ,5-didesoxy-D-glucit (ie 1 - deoxynojirimycin ,1-deoxynojirimycin, DNJ) [3]. After 1976, from mulberry (Morus spp.) Isolated moranoline (ie DNJ