Mulberry stalk Extraction and Determination of Polysaccharide.doc

 PAGE \* MERGEFORMAT 15 Mulberry stalk Extraction and Determination of Polysaccharide 【Abstract】 Objective To establish a determination of polysaccharides in mulberry stalk method. Methods of water extraction and alcohol precipitation of the mulberry stalk of the polysaccharides extracted with Sevag law purified anthrone - sulfuric acid content determination of polysaccharides. The results of the regression equation: A = 0.024 94C 0.057 1, r = 0.999 5, the linear range of 0.08 ~ 0.4 μg * L-1. Mulberry stalk polysaccharide content of 18.74% (RSD = 2.1%), the average recovery was 98.6%, RSD = 1.4%. Conclusion The method is simple, rapid and reproducible for the mulberry stalk provides a method of quality control. Keywords: mulberry stalk; polysaccharide;  concentrated sulfuric acid anthrone Abstract: ObjectiveTo establish a kind of determining method of contents of polysaccharides in Mulberry Twig. MethodsThe polysaccharide in Mulberry Twig was extracted with water and ethanol precipitation. Anthrone-sulfonic acid was applied to analysis of the contents of polysaccharides. ResultsThe content of polysaccharides in Mulberry Twig was 18.74 mg / g, average recovery was 98.6%, RSD = 1.4%. ConclusionThe results showed that this method is simple, specific and accurate. It provides a method for mass control of Mulberry Twig. Keywords:: Mulbery Twig; Polysaccharide; Anthrone-sulfonic acid method Moraceae mulberry stalk of the plant mulberry Morus alba L. shoots, Mulberry Twig mulberry stalk: also known as Sang article mulberry stalk has a long history of medicinal, “Chinese Pharmacopoeia” recorded as rheumatism, Lee joints commonly used drugs for the Treatment of Jianbi joint pain numbness [1]. Clinical application joint swelling and pain, hand, foot numbness, rheumatism Bi Tong, paralysis and other diseases. Polysaccharide is one of a variety of herbal active ingredients, polysaccharides have a very wide range of biological activit