N-acetylcysteine on acute lung injury in rat lung water channel protein expression.doc

 PAGE \* MERGEFORMAT 15 N-acetylcysteine on acute lung injury in rat lung water channel protein expression [Abstract] Objective: To observe the N-acetyl-cysteine (NAC) on acute lung injury (ALI) lung tissue -1 water channel protein expression, and further explore the N-acetylcysteine on acute lung injury (ALI) lung organization’s protection. Methods: Lipopolysaccharide (LPS)-induced acute lung injury (ALI) model, and then apply the N-acetyl-cysteine to intervene. Experimental set up the control group, model group, N-acetyl cysteine group, the lung injury model of rats were killed 24 h after the success, take the left lobe lung tissue. Using immunohistochemical staining to detect N-acetylcysteine on acute lung injury in rat lung tissue water channel protein -1 (AQP-1) expression, using HPIAS-2000 image analysis system measured the water channel protein -1 in the expressed in the above groups, the average optical density and the average positive area ratio. Results: The experimental control group expressed high levels of lung AQP-1; model light yellow staining in lung tissue, AQP-1 expression in the lower; N-acetyl cysteine group of lung tissue expression of high levels of AQP-1. The q test test between the control group and model group, AQP-1, the average optical density and the positive area ratio, a significant difference (P amp;lt;0.01), experimental control group and between groups of N-acetyl cysteine, AQP -1, the average optical density and the positive area was no significant difference in the rate (Pamp;gt; 0.05). Conclusion: N-acetylcysteine on acute lung tissues can AQP-1 was highly expressed on acute lung injury has an important protective role in the organization. [Keywords:] N-acetylcysteine on lung injury induced by water channel protein -1 Acute lung injury (ALI) is characterized by a large number of significant protein-rich exudate into the alveolar space, seriously affect the lung ventilation function, leading to hypoxemia, respiratory fail