NAA on K562 cells and normal human peripheral blood lymphocytes Comparison of effects of genetic material damage.doc

 PAGE \* MERGEFORMAT 4 NAA on K562 cells and normal human peripheral blood lymphocytes Comparison of effects of genetic material damage Authors: Lei Ni, Song-born, Jiang-Fang Lian, Wang Ai-ying, Liu Liying, Chen Huang [Abstract] Objective To compare the NAA (naphthalene acetic acid, NAA) on the K562 cells and normal human peripheral blood lymphocyte chromosome damage effects of differences. Methods The Preparation of sister chromatid exchange (SCE) and micronucleus testing NAA on K562 cells and normal human peripheral blood lymphocytes SCE, micronucleus formation rate as well as the mitotic index effects. The results of NAA can induce K562 cells and normal human lymphocyte micronucleus formation and improve the SCE frequency, as compared with the control group there was significant difference; NAA-induced K562 cells, micronucleus formation rate and the absolute increase in SCE frequency is higher than the normal lymphocytes in group . NAA significantly inhibited K562 cells and normal human lymphocytes mitotic index, absolute inhibition rate of K562 cells also showed higher than normal lymphocytes. Conclusion NAA can induce chromosome damage. [Keywords:] NAA; K562 cells; lymphocytes; SCE; micronuclei NAA (naphthalene acetic acid, NAA) as a plant growth hormone is widely used in plant tissue culture, as well as cultivation of crops such as soilless culture industry. Naphthalene on the human body has proven to have significant side effects, can cause chromosome aberrations [1]. Some naphthalene derivatives, such as naphthalene amines, can cause occupational bladder cancer; while others naphthalene derivatives can be used for clinical treatment, such as naphthalene-Fen is used to treat skin diseases [2]. This study group showed that NAA can inhibit the proliferation of K562 cells [3], but the NAA can be cancer treatment drugs, still need to be the genetic toxicological analysis. In this paper, NAA, as well as dealing with normal human peripheral blood lymphoc