Naja atra muscarinic separation of peptides and its implications for the thrill of effect of muscarinic receptors M.doc

 PAGE \* MERGEFORMAT 21 Naja atra muscarinic separation of peptides and its implications for the thrill of effect of muscarinic receptors M Authors: Chia-Bing, Huang Lifeng, Xu Ying, YU Chang-xi Keywords: cobra venoms;, receptors, cholinergic;, muscarinic;, cholinergic agonists;, radioactive receptor assay Abstract: Objective Naja atra Naja atra venom was isolated muscarinic peptides and explore its relationship with the M cholinergic receptors. Methods The molecular sieve gel Sephadex G50, Sephadex G100, ion-exchange gel CMSepharose FF component separation and purification purposes; SDSPAGE identification of the target protein purity and determine their relative molecular mass; radio-receptor method for determination of the substance in rat brain tissue M choline receptor affinity; selection of isolated guinea pig ileum longitudinal muscle to observe its effect on the M cholinergic receptors. Results from Naja atra venom in the rat brain tissue to obtain an M cholinergic receptor peptides with high affinity (Ki of 10.12 nmol / L), its relative molecular mass of approximately 14 kD. The peptide can cause isolated guinea pig ileum longitudinal muscle contraction, this effect can be blocked by atropine. Conclusion Naja atra venom obtained with the M cholinergic receptors with high affinity peptides, the peptide of M has the emotional effect of cholinergic receptors. Keywords: Cobra venoms; receptors, cholinergic; muscarinic; cholinergic agonist; radio-receptor assay ABSTRACT: Objective To isolate muscarinic peptide (MP) from Naja atra venom and to study the effect of MP in muscarinic cholinergic receptor (mChR). Methods MP had been isolated and purified from Naja atra venom by gel filtration on Sephadex G50 and Sephadex G100, chromatography on ionexchanger CMSepharose FF. Homogenicity and molecular weight of the MP was determined on SDSPAGE. Isolated ileal longitudinal muscles of the guinea pigs were used to observe the effect of MP