Naodesheng soft capsule of high performance capillary electrophoresis fingerprints_0.doc

 PAGE \* MERGEFORMAT 10 Naodesheng soft capsule of high performance capillary electrophoresis fingerprints Of: Wang Guangzhong, Yan-Ju Liu, Zhang, Xiao Jin, Liu Yan Wen [Abstract] Objective capillary electrophoresis (HPCE) method Naodesheng fingerprint of the capsule. METHODS running buffer: 30 mmol / L borax -50 mmol / L disodium hydrogen phosphate, pH9.98; operating voltage of 15 kV The detection wavelength was 260 nm, column temperature 25 ℃, using pressure injection, injection time, 8 s. analysis time 25 min. Results indicated a fingerprint of the 16 peaks, the peak area is greater than the total peak area of #8203;#8203;90 % and the method precision, stability and repeatability are in line with the relevant provisions of fingerprints. using the cosine method, calculation of 10 soft capsules Naodesheng approved fingerprint similarity results were between 0.93 to 1.00, but different manufacturers Naodesheng films were quite different. Conclusion The method Naodesheng the quality control provided. [Keywords:] Naodesheng soft capsule; fingerprint; high performance capillary electrophoresis Abstract: ObjectiveTo develop the fingerprint of Naodesheng soft capsules by HPCE.MethodsThe background electrolyte consisted of 30 mmol / L sodium borate and 50 mmol / L sodium hydrogen phosphate and its pH was 9.98, applied voltage was 15kV, operating temperature was 25 ℃, the detection wavelength was at 260 nm and the analysis time was 25min.Results16 peaks were indicated.The peak area of #8203;#8203;these components was over 90% of total area in the fingerprint.RSD of precision and reproducibility was in the range of 5%. The results of similarity evaluated by cosine were 0.93 ~ 1.00 from 10 batches, but that of 3 batches of Naodesheng tablets from the other manufacturers were different. ConclusionThe method may provide reliable foundation of quality control of Naodesheng preparation. Keywords:: Naodesheng soft capsules; Fingerprint; HPCE Naodesheng too