nell1 direct in vivo gene transfection of canine mandibular defect repair.docVIP

 PAGE \* MERGEFORMAT 11 nell1 direct in vivo gene transfection of canine mandibular defect repair [Abstract] Objective: To explore the gene-containing retrovirus Nell  1 fluid and poly-hydroxybutyrate (PLGA) composite biological materials to repair the mandibular defects in dogs. Methods: To prepare with Nell  1 eukaryotic expression vector reversal retroviral liquid PT  PLNCX2  Nell  1. Preparation of Beagle mandible 15 mm * 10 mm * 5 mm of box-like defect model, repair, test is divided into two groups: PT  PLNCX2  Nell  1 PLGA repair group (experimental group), PLGA repair group (control group), n = 8 samples, respectively, after 8,16 wk for gross observation, X ray detection and histological test. Results: PT  PLNCX2  Nell  1 recombinant virus liquid and Bio-Materials Conservation Section can be seen in the bone defect with bone formation of connections, bone defects, histological observation see a large number of new bone formation; PLGA repair group was no bony connection formed only part of the two broken bone defect is formed a small amount of bone tissue. Conclusion: The use of Nell  1 gene in vivo partial direct transfer methods can be used to repair mandibular defects in dogs. [Keywords:] Nell  1 gene; bone defect; gene transfection 0 Introduction In recent years, gene transfer technology in bone tissue regeneration, bone defect study of the role of more and more attention will be related to transfer of growth factors in vitro or in vivo method to the direct transfer of new bone tissue. Nell  1 is a kind of strong into the active bone growth factor, osteogenic capacity has been confirmed [1]. using retroviral-mediated approach can be Nell  1 protein into canine bone marrow mesenchymal stem cells (BMSc), after winning an exogenous Nell  1 expression at the same time, BMSc of the osteogenic activity has also been improved significantly. but Nell  1 gene contains a retrovirus-induced fluid whether in vivo osteogenic