Neonatal HBV intrauterine infection in the digestive tract Ways.doc

 PAGE \* MERGEFORMAT 2 Neonatal HBV intrauterine infection in the digestive tract Ways [Keywords:] Newborn [Keywords:] newborn; vertical transmission; hepatitis B 0 Introduction China and other Southeast Asian countries, high incidence of chronic hepatitis B, about 30% of HBV infections are transmitted through mother to child transmission access, and can not be combined active and passive immunization for prevention, the study of risk factors for HBV intrauterine infection of hepatitis B epidemic in China for the control of great significance to . An object and method 1.1 Object 200210/200310 continuous collection of Maternal and Child Health Hospital of Shaanxi Province HBsAg-positive pregnant women and their newborns 58 cases were studied to exclude HBsAg-negative pregnant women, 2 cases of neonatal serum or gastric juice inadequate or are not suitable for ELISA, three cases, so the actual number of cases included in for the 53 cases, pregnant women, average age (27.4 ± 3.5) years of age, newborn body weight (3.0 ± 0.9) kg, male 27 cases, 26 cases of female infanticide, no deformed children. Venous blood of pregnant women collected elbow, newborn within 24 h of birth, immunization to prevent venous blood before the shares, separation of serum, -20 ℃ to save backup. Taken within 1 h after birth, gastric juice, -20 ℃ to save backup. Blood after routine injection of hepatitis B immunoglobulin proteins and hepatitis B vaccine. HBsAg, HBeAg detection kit purchased in Shanghai Branch China Bio-technology companies. 1.2 Methods Serum, gastric juice HBsAg, HBeAg test: are used enzyme-linked immunosorbent assay (ELISA, Shanghai Branch China), the detection steps and the results of judging according to the instructions, the results of enzyme-linked by the automatic detector (Multiskan MK3) read out. Neonatal Unit HBsAg-positive blood to determine the intrauterine infection. Statistical analysis: Using SPSS 10.0 software, using χ 2 test or Fishers test ex