Neuronal apoptosis in the spinal cord ischemia-reperfusion injury in the role of delayed paralysis.doc

 PAGE \* MERGEFORMAT 8 Neuronal apoptosis in the spinal cord ischemia-reperfusion injury in the role of delayed paralysis Author: Ma Wei, Liu Bi-Bo, Liu Miao, Wang multi-ning, the party victory [Abstract] Objective To study the role of apoptosis in spinal cord ischemia-reperfusion injury is delayed paralysis in the role. Methods 48 New Zealand white rabbits were randomly divided into two groups: control group (sham) and ischemic-reperfusion group (IR). And to improve the way the light Zivin rabbit lumbosacral spinal cord ischemia-reperfusion model of delayed paralysis, comparing animals of each group at different time points hindlimb motor function and pathological changes; DNA in situ terminal deoxy-mediated enzyme transfer of urine triphosphate (dUTP) labeling method (TUNEL method) to detect the level of neuronal apoptosis. The results of HE staining showed that reperfusion 8h normal nerve cell morphology, structure, clearly there is a small amount of gray matter in the vacuoles, but the structural integrity of nerve cells; reperfusion 24h Group: gray matter in the anterior horn cells in severely damaged neurons, vacuolization , no significant infiltration of inflammatory cells; reperfusion 72h Group: a large number of vacuoles in the anterior horn gray matter in the formation of several structures still remaining clear motor neurons, there are clear infiltration of inflammatory cells; reperfusion 168h Group: gray matter in the motor neuron loss, pyknosis necrosis of a few remaining neurons. TUNEL staining method, sham group and 8h after reperfusion, only see the non-specific staining. Reperfusion a large number of positive cells after 24h to 72h reperfusion the number of positive cells reached a peak, mainly in the former horn motor neurons. Reperfusion 1 week after the gray matter of serious structural damage, only a small number of neurons survived, but its integral absorbance values of positive cells were still higher than the average control group. Con