New cell line ketones ANY2 on rat preadipocytes and lipid metabolism.doc

 PAGE \* MERGEFORMAT 14 New cell line ketones ANY2 on rat preadipocytes and lipid metabolism [Abstract] Objective To study the new cell line ketones ANY2 on rat preadipocytes and lipid metabolism. Methods collagenase digestion by primary rat preadipocytes were seeded in 24-well cell culture plate, to induce differentiation induction medium, oil red O staining compounds ANY2 on preadipocyte differentiation, and oil red O with isopropanol extraction measured absorbance, the degree of differentiation of each group. high concentrations in Dexamethasone effects on the differentiation of fat cells, insulin resistance model prepared to observe the compound on insulin resistance in fat cells ANY2 glucose consumption (GC) and free fatty acids (FFAs) the amount of overflow. Results concentration of 1.1mg L-1 compounds ANY2 can significantly promote preadipocyte differentiation, the differentiation rate of up to 152%, increase their fat storage capacity, increasing the number of small fat cells, improve insulin resistance in fat cells of sugar consumption reached 363 mg mg pro-1, reduce the free fatty acid overflow , overflow is only 69 mmol mg pro-1. Conclusion Compound ANY2 can promote primary preadipocyte differentiation filling fat, and improve the high concentration of dexamethasone caused by insulin resistance. [Keywords:] primary preadipocytes, differentiation, fatty acid spill, insulin resistance Effect of Novel Thiazolidinedione ANY2 on Glycometabolism and Lipid Metabolism in Primary Precursor Adipose Cells Abstract: Objective To investigate the effects of a novel thiazolidinedione ANY2 in primary precursor adipose cells.Methods 1.Using enzyme-digesting method, primary precursor adipose cells from the rat adipose tissues were cultured in 24wells Culture Plate.Using culture medium to induce the transformation of the cells and stained with oil red O to contrast the differentiation.2.Differentiaed cells were exposed to high concentration of dexamet