Non-contact co-culture in vitro differentiation of bone marrow mesenchymal stem cells to alveolar epithelial cell differentiation.doc

 PAGE \* MERGEFORMAT 18 Non-contact co-culture in vitro differentiation of bone marrow mesenchymal stem cells to alveolar epithelial cell differentiation Of: Wang Yan, Yang Zhijun, He Xi Yu, Zhi-Chun Feng [Abstract] Objective To establish a non-contact co-culture model in vitro and induce bone marrow mesenchymal stem cells (MSCs) to the alveolar epithelial cell differentiation. Methods divided into three groups, each with 6 cases. The control group: mice MSCs cultured alone group, Experimental group A: normal lung tissue single cell suspension + MSCs, the experimental group B: lung tissue damage single cell suspension + MSCs. incubated 8d, laser confocal and RT-PCR, co-culture system in the lower chamber SP-C and AQP5 expression. Results In the control group and experimental group A only detected AQP5, the experimental group B can simultaneously detect AQP5 and SP-C. experimental group B, the expression of AQP5 mRNA were markedly increased (P lt; 0.01), compared with the experimental group A, the AQP5 mRNA expression also significantly different (P lt;0.01). However, the experimental group compared with the control group A no significant statistical difference (Pgt; 0.05). Conclusion The room non-contact was successfully established in vitro differentiation of MSCs induced by the experimental model of alveolar epithelial cells. [Keywords:] bone marrow mesenchymal stem cells, alveolar epithelial cells, confocal ChinaABSTRACT: Objective To establish the co-culture model in vitro and induce bone marrow mesenchymal stem cells (MSCs) to differentiate into lung alveolar epithelial cells. Methods Each group had 6 samples, control group was MSCs alone; Group A was the MSCs cultured with the cells from normal lung; and Group B was the MSCs with the cells from injuried lung. Each group was cultured for 8 days and the two markers of lung alveolar epithelial cells including AQP5 and SP-C were tested by laser confocal microscopy and RT- PCR. Results Only AQP5 wa