NKG2D-mediated NK cell killing effect of nasopharyngeal carcinoma cells in vitro.docVIP

 PAGE \* MERGEFORMAT 2 NKG2D-mediated NK cell killing effect of nasopharyngeal carcinoma cells in vitro Author: Mei users to switch, Guo Yuan, WEI Hong-Mei, Chang Hong, Song Chaoyang [Abstract] Objective To investigate the CNE2 nasopharyngeal carcinoma cell-surface molecules HLA  I kind of phenotype and the expression of NKG2D ligands, to further understand their effects on allogeneic NK cell killing activity. Method of flow cytometry NKG2D ligands MICA, MICB, ULBP1, ULBP2, ULBP3 in the K562, CNE2 cell expression. PCR  SSP Analysis CNE2 cells in HLA  A, B, Cw typing and NK cell KIR genotyping. Determination of LDH release of 5 cases of healthy NK cells in various effector-target ratio when K562, CNE2 cell-killing activity, efficiency observed target ratio 20:1, monoclonal anti-NKG2D ligands on NK cell killing K562, CNE2 cell activity impact. Results CNE2 cells expressed MICA, MICB, ULBP2, did not express ULBP1, ULBP3. K562 cell surface expression of MICA, MICB, ULBP1, ULBP2, ULBP3. 5 healthy persons NK cell inhibitory KIR and CNE2 cell surface HLA  I type there is a mismatch between molecules. Effect when the NK cell-target ratio 5:1,10:1,20:1,30:1 right K562, CNE2 cell-killing activity, respectively (29.02 ± 0.45)%, (10.50 ± 2.17)%; (44.43 ± 1.36) %, (27.68 ± 1.47)%; (57.82 ± 1.35)%, (36.99 ± 3.13)%; (71.24 ± 2.36)%, (55.00 ± 2.20)%, when the target ratio in each effect on K562 cells, NK cells, anti - markedly enhanced activity compared with CNE2 cells (P = 0.000); the effect target ratio 20:1, anti  MICA, anti  MICB, anti  ULBP1, anti  ULBP2, anti  ULBP3 could inhibit NK cell killing activity of K562 cells , blocking the former compared with the significant difference (P = 0.000); anti  MICA, anti  MICB, anti  ULBP2 could inhibit NK cells CNE2 cell-killing activity, blocking the former compared with the significant difference in (P