Non-radioactive marker BrdU Research.doc

 PAGE \* MERGEFORMAT 14 Non-radioactive marker BrdU Research Bromodeoxyuridine (bromodeoxyuridine, BrdU) is a DNA precursor thymidine analogue, incorporation of S-phase cells by competitive single-stranded Alternative thymine DNA nucleotide sequence. BrdU and thymidine incorporation of the intensity of competition may be related to abnormal cell proliferation, such as liver cells, approximately 11.6% of the thymine is replaced, while the normal liver cells, only 1.1% [1]. Past research in molecular genetics are often used in isotope-labeled, but there are radioactive contamination, technical difficulties, experimental and long-cycle defects. In recent years, non-radioactive marker for research and development rapidly, but its sensitivity is less than the majority of isotopes, so that experimental use is limited. Gratzer was prepared from 82 years of anti-BrdU monoclonal antibody and marker detection technology continues to improve to improve the application has the sensitivity of BrdU labeled deuterium thymidine (3H-TdR) similar to [2,3]. BrdU is currently considered the most promising to replace the non-radioactive isotope is one marker. This paper reviews the recent BrdU Research, focusing on technical issues related to the tagging process. 1, marking technology: 1, doses, and means: BrdU in vivo or in vitro can be used to mark the body tag, the mice according to the weight 60mg-200mg/kg peritoneal injection in rats [4,5], dogs by intravenous injection of 100mg/kg body weight [6], the human body to the surface area 150μ g/m2 dosage, take samples before the intravenous injection of 8.25-10h [7], immunohistochemical staining. After intravenous administration of BrdU in general no side effects, occasional reports of excessive can cause cell mutation. In vitro markers in a broad and deep, usually stored in the fresh tissue culture medium shear spit to the 1-2mm3 size, removed the supernatant after centrifugation, add 100μ g/mlBrdU, or fresh tissue co