No sieve type Preparation of solid phase extraction column and the determination of benzoic acid in food research.doc

 PAGE \* MERGEFORMAT 13 No sieve type Preparation of solid phase extraction column and the determination of benzoic acid in food research Study: Song Wenqing Liu Yaxiong Jianmin Wang Qin package [Abstract] combined with solid phase extraction (SPE) disk and supports the SPE column with technology, preparing a new type of non-sieve-type solid phase extraction column. To C18 packing, for example, to plum for the media on which the sample acid analysis and by traditional solid-phase extraction column parallel comparison; the combination of SPE and HPLC UV was investigated in acid medium for simple filling the maximum adsorption and desorption curves of the new SPE column in the practical application isolation and purification. The results show that the new SPE column better absorption of the sample, the specification for the 200 mg / 3 mL of SPE columns for the adsorption of benzoic acid to 0.951 mg, more than the traditional column adsorption capacity 0.908 mg; the wash off the curve almost coincides with the traditional column; benzoic acid in 1 ~ 100 mg / L concentration range a good linear relationship, r = 0.9999, with the SPE column after purification the recovery rate and the relative error of 88.4% ~ 102.3% in and 1.4% -2.9%. [Keywords:] solid phase extraction column, sieve, packing, acid 1 Introduction Solid phase extraction (SPE) is a sample pretreatment by liquid-solid extraction and liquid chromatography technology developed from the combination of [1]. As a liquid sample preparation methods, SPE is better than traditional liquid-liquid extraction ( LLE), if no emulsification; not need to use ultra-pure solvents, low organic solvent consumption, environmental pollution; for small sample size; treated sample rate, and particularly suitable for dealing with volatile and thermally unstable drugs on [ 2,3]. SPE SPE column is the most common forms of use, the general SPE column is similar to the syringes of plastic or glass column filled t