Ning brain damage on cerebral ischemia-reperfusion injury in rats.doc

 PAGE \* MERGEFORMAT 6 Ning brain damage on cerebral ischemia-reperfusion injury in rats [Abstract] Objective To observe the brain injury rather on cerebral ischemia-reperfusion brain injury in rats. Methods rats with bilateral vertebral artery coagulation closed 4 ~ 5 h after the occlusion of common carotid artery 30 min, reperfusion 90 min, caused by cerebral ischemia-reperfusion injury in rat model of brain injury observed in brain tissue rather biochemical markers and brain electroretinogram impact. The results of ischemia-reperfusion injury caused by severe brain tissue, brain tissue water, Na, Ca2 content and malondialdehyde (MDA) content was significantly higher than the control group, while the superoxide dismutase (SOD) activity lower than the control group, and associated with abnormal EEG and brain pathological changes in the organizational structure. Common carotid artery occlusion 30 min before the intraperitoneal injection of brain injury can reduce the brain tissue-ning water, Na, Ca2 content and MDA content, and increase SOD activity, for histological examination and EEG also improved. Conclusion brain injury rather on cerebral ischemia-reperfusion injury has a protective effect. [Keywords:] brain injury Ning; cerebral ischemia; reperfusion injury; Ca2 overload Abstract: Objective To study the protective effects of Naoshangning recipe on brain injury in rats.Methods The bilateral vertebral arteries of rats were occluded, and 4 ~ 5 h later, the carotid arteries of rats in the conscious condition were occluded for 30 min followed by reperfusion for 90 min.The effects of Naoshangning recipe on the contents of water, Na, and Ca2 in cortex, malondialdehyde (MDA) content and superoxide dismutase (SOD) activity in brain hemispheres, as well as the EEG activities were observed.Results 30 min ischemia and 90 min after reperfusion, the contents of water, Na, Ca2, and MDA were increased, and the SOD activity was reduced with abnormal EEG activity and is