Newcastle disease virus on the inhibition of blood vessel growth.doc

 PAGE \* MERGEFORMAT 10 Newcastle disease virus on the inhibition of blood vessel growth 【Abstract】 Objective To study the Newcastle disease virus on the blood vessel growth inhibition. Methods The mouse model of lung metastasis observed Newcastle disease virus (NDV) on cancer metastasis and the growth of capillaries within the organization; in vitro drug sensitivity test (MTT) detection of NDV in different doses and action time under human vascular endothelial cells ECV304 inhibitory effect on cell proliferation; with PI, and Hoechst33342 the role of fluorescence staining of cell availability of NDV and the morphological changes of apoptotic changes. The results of NDV in mice in vivo can effectively inhibit metastasis occurs when the tissue microvessel formation in vitro can inhibit the proliferation of ECV304 cells and lead to relatively minor apoptosis. Conclusion vitro and in vivo experiments show that NDV can inhibit the growth of tumor microvessels occurs, the role of vascular endothelial cells by inhibiting the proliferation rather than direct cytotoxicity achieved, the mechanism may be related to a specific viral protein related, to be furtherResearch. Keywords: NDV; angiogenesis inhibitor; lung metastasis Growth Inhibition of Newcastle Disease Virus (NDV) on Vein Keywords:: NDV; Angiogenesis inhibitor; Pulmonary metastasis Tumor growth dependent on the growth of the environment to obtain nutrients from the environment to the excretion of metabolites, when the amount of tumor cells is more monkeys are numerous, their exchange of matter between environment and can be done by way of diffusion, however, when the tumor tissue increased to a certain extent when the exchange of material necessary to pass the intervening vascular network to complete. Studies have shown that the growth rate of tumor tissue and its dependence on the blood supply is proportional to. Therefore, theoretically speaking, by inhibiting blood vessel gro