Normal phase silica gel - select eluting gas chromatography liquid chromatography mass spectrometry.doc

 PAGE \* MERGEFORMAT 27 Normal phase silica gel / select eluting gas chromatography liquid chromatography mass spectrometry Of: Zhang Jinhu Lin Yonghui Liang Zhen, Liu Jianjun [Abstract] the normal phase silica gel / select eluting at the core, the establishment of a complex matrix for methamidophos residues in food before treatment. Samples with ethyl acetate anhydrous Na2SO4 homogeneous grinding, ultrasonic assisted extraction, extract powder dispersed by the PSA and the LC Si SPE column eluting purified single solvent selected, gas-phase chromatography (GC) and ultra-high performance liquid chromatography tandem mass spectrometry (UPLC MS / MS) analysis. gas chromatography The flame photometric detector (FPD), liquid chromatography electrospray ionization mass spectrometry using positive ion mode (ESI +), T3 bonding technology (HSS T3) and hydrophilic interaction (HILIC) ultra-high performance liquid column chromatography with external standard quantification. The method is simple, rapid, and by optimizing the pre-treatment conditions on the machine, tested under optimal conditions, gas chromatography, liquid chromatography mass spectrometry the limit of quantification (S / Ngt; = 10) was 0.0007 ~ 0.006 mg / kg, recoveries were 73% to 90%, 81% -96%, relative standard deviation of 2.4% to 6.1%, 5.2% to 10.8%. and select the elution mechanism of the purification process was study. [Keywords:] gas chromatography, ultra performance liquid chromatography tandem mass spectrometry, T3 bonding technology chromatography, residue analysis, methamidophos 1 Introduction Methamidophos (Methamidophos), is an efficient, broad-spectrum organophosphate insecticide and miticide, was the largest production and use of pesticides [1], although the state in 2007 banned the sale and use of However, repeated violations of methamidophos residues exceeding and food poisoning incidents occur, making the detection of methamidophos residue issue has become the focus of at