Nourishing spleen yin herbs containing serum internal endoplasmic reticulum stress-protective effect of neuronal damage and its mechanism.doc

 PAGE \* MERGEFORMAT 19 Nourishing spleen yin herbs containing serum internal endoplasmic reticulum stress-protective effect of neuronal damage and its mechanism Author: War-li-bin, ZHONG Jun-hua, Lu Xiao-Guang, SUI Hua, Wei Wei [Keywords:] endoplasmic reticulum; tunicamycin; glucose-regulated protein 78; apoptosis-promoting factor CCAAT / enhancer binding protein homologous protein; mice Endoplasmic reticulum (endoplasmic reticulum, ER) widely present in eukaryotic cells, is protein synthesis, folding, transport and intracellular calcium ions stored in the main sites. The endoplasmic reticulum calcium ion disorder and can lead to accumulation of unfolded proteins within the endoplasmic reticulum stress (endoplasmic reticulum stress, ERS), occurred with a protective effect of unfolded protein response (unfolded protein response, UPR). Endoplasmic reticulum stress directly affect the stress transferred to cells, such as repair, damage, or apoptosis. Nervous system of many diseases and endoplasmic reticulum stress-related. Recent studies have shown that endoplasmic reticulum stress-mediated apoptosis signal transduction pathway may be related to Alzheimer’s disease (Alzheimer disease, AD) pathogenesis. This study concept Chazi Spleen Yin herbs (Zibu Piyin Recipe, ZBPYR) serum-containing sugar chains by the N  inhibitor tunicamycin (tunicamycin, Tm) due to the impact of endoplasmic reticulum stress to explore the neural protection mechanisms. 1 Materials and methods 1.1 The experimental drug prescriptions by nourishing spleen yin Qing Wu Cheng ‘no home assembly’ funded Cheng Tang flavored made from red ginseng 30 g, yam 15 g, Poria 15 g, Radix Paeoniae Alba 15 g, Salvia 12 g, white beans 15 g, lotus meat 20 g, Shichangpu 10 g, Polygalaceae 10 g, sandalwood 4.5 g, orange-red 9 g, licorice 9 g composition, were purchased from Dalian Medical Group medicine company. Conventional medicine decoction per ml of liquid containing crude drug 3.29 g, 4 ℃ to save back